How to Calculate Peptide Reconstitution

The Basic Formula

Reconstitution math is simpler than it seems. You just need to know:

• How much peptide is in the vial (in mg)
• How much water you're adding (in mL)
• What dose you want (in mcg or mg)

Step-by-Step Example

Given:

• Vial contains: 5mg of peptide
• Adding: 2mL bacteriostatic water
• Desired dose: 250mcg

Step 1: Calculate concentration

Step 2: Calculate volume needed

Step 3: Convert to syringe units

On a standard 100-unit insulin syringe:

• 1mL = 100 units
• 0.1mL = 10 units

Understanding Syringe Types

Insulin syringes come in different sizes:

Syringe	Total Volume	Each Unit
100-unit	1.0mL	0.01mL
50-unit	0.5mL	0.01mL
30-unit	0.3mL	0.01mL

Tip: Smaller syringes (30 or 50 unit) have finer markings, making it easier to measure small doses accurately.

Common Water Amounts

Here's a quick reference for different water amounts with a 5mg vial:

Water Added	Concentration	250mcg Dose
1mL	5,000mcg/mL	5 units
2mL	2,500mcg/mL	10 units
2.5mL	2,000mcg/mL	12.5 units

Pro Tips

• Use more water for easier measurement of small doses
• Use less water if you need to inject smaller volumes
• Double-check your math before every injection
• Track your vial usage to know when you're running low